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primary antibodies against mgmt  (Novus Biologicals)


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    Novus Biologicals primary antibodies against mgmt
    Primary Antibodies Against Mgmt, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against mgmt/product/Novus Biologicals
    Average 93 stars, based on 14 article reviews
    primary antibodies against mgmt - by Bioz Stars, 2026-05
    93/100 stars

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    U0126 enhanced TMZ-induced DNA damage. (A) Protein expression levels <t>of</t> <t>p-ATR,</t> γ-H2AX, p-ATM, p-ERK1/2 and ERK1/2 were detected when HepG2 cells were treated with TMZ (1 mM) and U0126 (4 and 8 µM) for 24 h by Western blot analysis (*, P<0.05, **, P<0.01 vs. the indicated treatment). TMZ, temozolomide. (B) Immunofluorescence staining analysis of HepG2 cells for γ-H2AX (red). DAPI (blue) was used to stain the nuclei (representative fields at ×200 magnification). TMZ, temozolomide; MAPK/ERK, mitogen-activated protein kinase/extracellular signal regulated kinase pathway; <t>MGMT,</t> methylguanine-DNA methyltransferase.
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    U0126 enhanced TMZ-induced DNA damage. (A) Protein expression levels <t>of</t> <t>p-ATR,</t> γ-H2AX, p-ATM, p-ERK1/2 and ERK1/2 were detected when HepG2 cells were treated with TMZ (1 mM) and U0126 (4 and 8 µM) for 24 h by Western blot analysis (*, P<0.05, **, P<0.01 vs. the indicated treatment). TMZ, temozolomide. (B) Immunofluorescence staining analysis of HepG2 cells for γ-H2AX (red). DAPI (blue) was used to stain the nuclei (representative fields at ×200 magnification). TMZ, temozolomide; MAPK/ERK, mitogen-activated protein kinase/extracellular signal regulated kinase pathway; <t>MGMT,</t> methylguanine-DNA methyltransferase.
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    U0126 enhanced TMZ-induced DNA damage. (A) Protein expression levels of p-ATR, γ-H2AX, p-ATM, p-ERK1/2 and ERK1/2 were detected when HepG2 cells were treated with TMZ (1 mM) and U0126 (4 and 8 µM) for 24 h by Western blot analysis (*, P<0.05, **, P<0.01 vs. the indicated treatment). TMZ, temozolomide. (B) Immunofluorescence staining analysis of HepG2 cells for γ-H2AX (red). DAPI (blue) was used to stain the nuclei (representative fields at ×200 magnification). TMZ, temozolomide; MAPK/ERK, mitogen-activated protein kinase/extracellular signal regulated kinase pathway; MGMT, methylguanine-DNA methyltransferase.

    Journal: Annals of Translational Medicine

    Article Title: Blocking MAPK/ERK pathway sensitizes hepatocellular carcinoma cells to temozolomide via downregulating MGMT expression

    doi: 10.21037/atm-20-5478

    Figure Lengend Snippet: U0126 enhanced TMZ-induced DNA damage. (A) Protein expression levels of p-ATR, γ-H2AX, p-ATM, p-ERK1/2 and ERK1/2 were detected when HepG2 cells were treated with TMZ (1 mM) and U0126 (4 and 8 µM) for 24 h by Western blot analysis (*, P<0.05, **, P<0.01 vs. the indicated treatment). TMZ, temozolomide. (B) Immunofluorescence staining analysis of HepG2 cells for γ-H2AX (red). DAPI (blue) was used to stain the nuclei (representative fields at ×200 magnification). TMZ, temozolomide; MAPK/ERK, mitogen-activated protein kinase/extracellular signal regulated kinase pathway; MGMT, methylguanine-DNA methyltransferase.

    Article Snippet: The filters were blocked using 5% nonfat milk (Mengniu, Beijing, China) at room temperature for 2 h. Primary antibodies against MGMT (CST; 1:2,000), p-ATR (CST; 1:1,000), p-ATM (CST; 1:1,000), γ-H2AX (CST; 1:1,000), and β-actin (CST; 1:10,000) were added to filters and incubated overnight at 4 °C.

    Techniques: Expressing, Western Blot, Immunofluorescence, Staining